Steroid isolation , depending on context, is the isolation of chemical matter required for chemical structure elucidation, derivitzation or degradation chemistry, biological testing, and other research needs (generally milligrams to grams, but often more  or the isolation of "analytical quantities" of the substance of interest (where the focus is on identifying and quantifying the substance (for example, in biological tissue or fluid). The amount isolated depends on the analytical method, but is generally less than one microgram.  [ page needed ] The methods of isolation to achieve the two scales of product are distinct, but include extraction , precipitation, adsorption , chromatography , and crystallization . In both cases, the isolated substance is purified to chemical homogeneity; combined separation and analytical methods, such as LC-MS , are chosen to be "orthogonal"—achieving their separations based on distinct modes of interaction between substance and isolating matrix—to detect a single species in the pure sample. Structure determination refers to the methods to determine the chemical structure of an isolated pure steroid, using an evolving array of chemical and physical methods which have included NMR and small-molecule crystallography .  : 10–19 Methods of analysis overlap both of the above areas, emphasizing analytical methods to determining if a steroid is present in a mixture and determining its quantity. 
- Roots considered antiasthmatic and stimulant.
- Leaves considered anodyne.
- Fruit considered cooling, digestive, phlegmatic.
Parts used and preparation
Fruits, roots, leaves, .
Edibility / Nutritional
- Fruit is an excellent vegetable and popular in the rural day-to-day cuisine. It is eaten before it ripens, preferred before the seed hardens.
- Also used in native pickles and curries in India.
- A good source of vitamins A, B, and C.
- A good source of calcium, phosphorus, and iron; carbohydrates and fiber.
- Decoction of roots taken internally for asthma and as a general stimulant.
- Leaves are used for piles.
- The boiled root of the wild plant, mixed with sour milk and grain porridge, has been used for the treatment of syphilis.
- Decoction of roots, dried stalk, and leaves is used for washing sores, exudative surfaces and used as astringent for hemorrhage from the bladder and other hemorrhagic fluxes.
- The juice of leaves used for throat and stomach troubles.
- Juice of the fruit, sometimes with pounded leaves, rubbed on suspected syphilitic eruptions of the hands.
- Fruit considered cooling, and bruised with vinegar
- Chinese and Annamites used the roots for skin diseases.
- The fruit is considered cooling, and bruised with vinegar, is used as a poultice for abscesses and cracked nipples.
- In Taiwan folk medicine, roots are used for rheumatism, inflammation and foot pain.
- Long fruit is phlegmatic and generative of phthisis, coughs, and anorexia.
- The peduncle, incinerated, used in intestinal hemorrhages, piles, and toothache.
- Seeds used as stimulant but may cause dyspepsia and constipation
- In French Guinea, decoction or infusion of leaves is used for stomach troubles and sore throat.
- In India , juice of various plant parts and pulp of fruits of S. melongena and its wild allies used for various ailments: diabetes, otitis, toothaches, cholera, bronchitis, asthma, dysuria, among many others.